Fig. 1 Amplification from different sample types

20% human whole blood containing anticoagulants (EDTA, Heparin and Citrate) and bovine whole blood (EDTA), human cerebrospinal fluid, human urine and cow whole milk were analyses along with 2% alkaline or proteinase K mouse tail, liver and brain crude DNA extracts. The results illustrate that the reaction efficiency of the SensiFAST Direct Probe SuperMix remained within tolerances (90-110%) in the presence of a wide range of common PCR inhibitors.

Fig.2 Amplification from biofluids

A 10-fold serial dilution of genomic DNA spiked into human urine was amplified, using SensiFAST Direct Probe SuperMix (green) and an Inhibitor-Tolerant Mix from supplier K (red) and the manufacturers’ recommended protocol. The results illustrate SensiFAST Direct qPCR Probe SuperMix is more sensitive than the mix from supplier K, as lower dilutions could be detected, with better efficiencies for all three inhibitor samples.

Fig. 3 Amplification from animal DNA in lysate solutions

A 10-fold serial dilution of genomic DNA from mouse tail clippings was spiked proteinase K lysate and after neutralization, used with SensiFAST Direct Probe SuperMix (green) and an Inhibitor-Tolerant Mix from supplier K (red) using the manufacturers’ recommended protocol. The results illustrate SensiFAST Direct qPCR Probe SuperMix is more sensitive than the mix from supplier K, particularly with the proteinase K lysate, as lower dilutions could be detected, with better efficiencies.