bioline/Proteinase K Solution/5 x 5ml/BIO-37085
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美元价：
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联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
Proteinase K is a highly active serine protease with broad cleavage specificity on native and denatured proteins. Proteinase K is widely used in the purification of native RNA and DNA from tissues or cell lines and many other applications.
Product Highlights
- High activity - broad-spectrum serine protease
- Active under denaturing conditions - useful for a variety of applications
- Stable - at high temperatures
- Molecular biology grade - useful for general digestion of proteins
- Proteinase K is also available in powder form
Product Description
Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is especially suitable for isolating PCR and RT-PCR templates.
The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and at elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/mL for protein removal and enzyme inactivation and up to 2 mg/mL for tissue treatment.
Proteinase K products are free of detectable DNase and RNase.
Applications
- Inactivation of RNases/DNases during nucleic acid extraction
- Protein modification
- General protein digestion
- Determination of enzyme localization
- Remove nucleases for in situ hybridization
- Improving cloning efficiency of PCR products
品牌介绍
Bioline成立于1992年，总部设在英国伦敦；随着时间推移，信息化的高速发展，Bioline目前已经成国际化公司，其分部设在美国，德国，法国和澳大利亚。BIOLINE是一家通过ISO9001:2008认证的公司，是世界上为数不多的超纯的dNTPs制造商之一。BIOLINE能够为大型实验室提供批量，定制和OEM的dNTP，我们已经开发和制造多种专利试剂和试剂盒。我们的核心竞争力，研发和制造的主要活动领域包括：实时定量PCR试剂盒，DNA聚合酶，逆转录酶，克隆试剂，核酸分离，超纯核苷酸合成，DNA与RNA分子量Marker，感受态细胞，分子生物学试剂，蛋白的表达及纯化。2010年，Meridian Life Science收购了 Bioline 公司。
